How does zinc stabilize the zinc finger?
Initially, the term zinc finger was used solely to describe DNA-binding motif found in Xenopus laevis; however, it is now used to refer to any number of structures related by their coordination of a zinc ion. In general, zinc fingers coordinate zinc ions with a combination of cysteine and histidine residues.
How do zinc fingers regulate transcription?
The zing-finger protein ZNF750 (C2H2-type, transcription factor) also acts as an essential regulator of keratinocyte differentiation. During epidermal differentiation, TP63 transcription regulates ZNF750 expression, which subsequently directly activates KLF4 expression.
What do zinc finger motifs interact with?
Zinc finger motifs encode both histidine and cysteine which directly coordinate zinc and are known to bind to specific DNA sequences and regulate DNA replication (Vallee & Auld, 1995).
How does zinc finger work?
Zinc-finger nucleases (ZFNs) are targetable DNA cleavage reagents that have been adopted as gene-targeting tools. ZFN-induced double-strand breaks are subject to cellular DNA repair processes that lead to both targeted mutagenesis and targeted gene replacement at remarkably high frequencies.
How do zinc finger nucleases work?
Zinc finger nucleases (ZFNs) are a class of engineered DNA-binding proteins that facilitate targeted editing of the genome by creating double-strand breaks in DNA at user-specified locations.
Is CRISPR better than ZFN?
The CRISPR/Cas technology offers a myriad of advantages over ZFN, as it relies on a single targeting molecule (guide RNA) for DNA sequence recognition. Another way in which construction of CRISPR plasmids is simplified in comparison to ZFN is that DNA recognition sites are composed of nucleic acid rather than protein.
What do you know about the zinc finger motif?
1 Organisation of Chromosomes. Christoph J. Feinauer. 2 Zinc Fingers. The zinc finger motif was discovered in 1985 by Miller, McLachlan, and Klug in a protein responsible for controlling gene expression – the Xenopus transcription factor IIIA (TFIIIA). 3 Mitochondrial Function. Sandra R. Bacman.
What is the consensus sequence of zinc finger?
A zinc-finger domain is characterized by the consensus sequence X3 -Cys-X 2–4 -Cys-X 12 -His-X 3. 5 -His-X 4 (where X is any amino acid residue), which forms a compact globular structure that contains a β sheet and an a helix held together by a central Zn ion.
How are zinc finger domains different from other domains?
Zinc finger (Znf) domains are relatively small protein motifs that contain multiple finger-like protrusions that make tandem contacts with their target molecule. Some of these domains bind zinc, but many do not, instead binding other metals such as iron, or no metal at all.
How is the fold group of the zinc finger defined?
This fold group is defined by two short β-strands connected by a turn (zinc knuckle) followed by a short helix or loop and resembles the classical Cys 2 His 2 motif with a large portion of the helix and β-hairpin truncated.
