How do you make a 100mm PMSF?

To prepare a 100 mM solution, add 17.4 mg of PMSF per milliliter of isopropanol. Store at −20°C. PMSF is inactivated in aqueous solutions. The rate of inactivation increases with increasing pH and is faster at 25°C than at 4°C.

How do I prepare PMSF stock?

How to make a 1 M PMSF Stock Solution

Weigh 1.74 g PMSF. 2 g. 00:03:00. PMSFP212121Catalog #RP-P20270.
Add DMSO to a final volume of 100 ml. Dissolve completely. 00:03:00.
Stocks may be kept at -20°C for up to 6 months. PMSF is used at a final concentration of 0.1- 1.0 mM. PMSFP212121Catalog #RP-P20270.

What is PMSF solution?

Phenylmethanesulfonyl fluoride (PMSF) is a general. inhibitor of serine proteases,2 such as chymotrypsin, trypsin, and thrombin. PMSF inhibits serine proteases. by sulfonating the hydroxyl groups of reactive-site.

How do you dilute PMSF?

Use. PMSF is not soluble in water, dissolve in isopropanol, ethanol or methanol. 200 mM stock solution (0.035 g/mL) is stable for months at 4°C, although storage at -20 is probably better. A common final concentration of PMSF is 1mM.

How long does PMSF last in solution?

PMSF is inactivated in aqueous solutions. The rate of inactivation increases with increasing pH and is faster at 25°C than at 4°C. The half-life of a 20 mM aqueous solution of PMSF is ~35 min at pH 8.0.

Where is PMSF stored?

Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, between the following temperatures: 20 to 25°C.

Is PMSF reversible?

Inhibition of the cysteine proteases by PMSF or AEBSF can be reversed by treatment with a sulfhydryl reagent such as DTT, but inhibition of serine proteases is irreversible.

Is PMSF irreversible?

It is an irreversible serine protease inhibitor, and is used at the same concentration as PMSF, but is nontoxic and stable in aqueous buffer solutions.

Why is PMSF toxic?

Phenylmethanesulfonyl fluoride (PMSF) is an acutely toxic and corrosive compound. PMSF hydrolyzes upon exposure to water liberating a toxic, acidic gas which in contact with metal surfaces can generate flammable and/or explosive hydrogen gas. Forms hydrofluoric acid upon exposure to water, which is extremely toxic.

How long is PMSF stable?

Stock solutions of PMSF in 100% isopropanol are stable for at least 9 months at 15 to 25 °C.

How toxic is PMSF?

Phenylmethanesulfonyl fluoride (PMSF) is an acutely toxic and corrosive compound. Toxic if swallowed; has a lethal dose of 50% of the population (LD-50 oral, mouse) of 200 mg/kg. It causes severe eye damage, including potential blindness. Forms hydrofluoric acid upon exposure to water, which is extremely toxic.

How long is PMSF good for?

I notice that this Sigma PMSF datasheet suggests a 3 year shelf life when properly stored (https://www.sigmaaldrich.com/content/dam/sigma-aldrich/docs/Sigma/Product_Information_Sheet/1/p7626pis.pdf).

How much PMSF is in a 200 mM solution?

No data images are currently available. For a 200 mM (200X) stock solution, reconstitute the 34.84 mg of lyophilized PMSF supplied in the product tube in 1 mL isopropyl alcohol. PMSF is unstable in water and should be added to lysis buffers or other aqueous solutions just prior to use.

How long can PMSF be stored in solution?

Once in solution, PMSF can be stored at -20ºC for up to 3 months, protected from light. Phenylmethanesulfonyl Fluoride (PMSF) is an inhibitor of serine proteases such as trypsin, chymotrypsin, thrombin, and papain. It is routinely added as a supplement to lysis buffers just prior to lysis, to prevent protease degradation.

Which is the best place to buy PMSF?

You can buy PMSF for example at Pierce or Sigma . PMSF is not soluble in water, dissolve in isopropanol, ethanol or methanol. 200 mM stock solution (0.035 g/mL) is stable for months at 4°C, although storage at -20 is probably better. A common final concentration of PMSF is 1mM.

How does Thermo Fisher Scientific’s PMSF work?

Thermo Scientific PMSF is a protease inhibitor that reacts with serine residues to inhibit trypsin, chymotrypsin, thrombin and papain. Features of PMSF: • Targets serine proteases, including trypsin, chymotrypsin, thrombin and papain. • Effective in most protein solutions at 0.1 to 1 mM final concentration.