What is anti rabbit IgG antibody?
Goat anti rabbit IgG antibody recognizes the Fc region of rabbit immunoglobulin G, confirmed by ELISA and immunoelectrophoresis. The antibody has not been species cross adsorbed and may cross react with IgG from other species. It shows weak recognition of rabbit IgM in ELISA.
What is the role of goat anti rabbit IgG HRP conjugate in ELISA?
Goat Anti-Rabbit IgG Antibody, HRP-conjugate detects level of Rabbit IgG & has been published & validated for use in WB, IH, ELISA. Goat Anti-Rabbit IgG Antibody, HRP-conjugate MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
What is the role of goat anti rabbit IgG horseradish peroxidase conjugate in the experiment?
Goat anti-Rabbit IgG Secondary Antibody [HRP (Horseradish Peroxidase)] Summary. Detects rabbit IgG heavy and light chains in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with human IgG, mouse IgG, and chicken IgY is observed.
What is Rabbit IgG?
Rabbit IgG is an isotype control used to estimate the non-specific binding of target primary antibodies due to Fc binding or other protein-protein interactions.
What is HRP conjugate?
Horseradish peroxidase (HRP) is a 44 kDa glycoprotein with 6 lysine residues, which can be conjugated to antibodies and proteins for use in a variety of applications. HRP is a popular detection label used in research. Antibody-HRP conjugates are commonly used in ELISA, IHC, and western blotting.
What is an HRP conjugate?
What is the substrate for HRP?
Colorimetric substrates for ELISA applications
| Substrate | Enzyme | Absorbance and color |
|---|---|---|
| OPD Substrate | HRP | 490 nm (450 nm) Green (Orange) |
| PNPP (p-Nitrophenyl Phosphate) | ||
| 1-Step PNPP Substrate Solution | AP | 405 nm Yellow |
| PNPP Substrate | AP | 405 nm Yellow |
How is anti mouse IgG HRP conjugate generated?
Polyclonal secondary antibody raised in goat, immunoaffinity-purified using immobilized antigens and conjugated to horseradish peroxidase (HRP) enzyme. The Anti-Mouse IgG (H+L), HRP Conjugate, antibody binds to both heavy and light chains for all mouse IgG subclasses.
Which is the best goat antibody for rabbit IgG?
Goat Anti-Rabbit IgG Antibody, HRP-conjugate detects level of Rabbit IgG & has been published & validated for use in WB, IH, ELISA. Highly purified whole rabbit IgG. Specific for rabbit IgG heavy and light chains. Evaluated by western blot. Western Blot Analysis: Suitable for western blotting (dot blot), ELISA and immunohistochemistry.
Can a goat IgG antibody be used in Elisa?
Goat Anti-Rabbit IgG Antibody, HRP-conjugate detects level of Rabbit IgG & has been published & validated for use in WB, IH, ELISA. More>> Goat Anti-Rabbit IgG Antibody, HRP-conjugate detects level of Rabbit IgG & has been published & validated for use in WB, IH, ELISA. Less<
How to make horseradish peroxidase IgG antibody for goats?
Goat polyclonal conjugated with horseradish peroxidase IgG in buffer containing 50% storage buffer 0.02 M potassium phosphate, 0.15 M NaCl, pH 7.2, 10 mg/mL BSA, 0.01% (w/v) gentamicin sulfate. Rehydration: Add 500 µL sterile, distilled water to make a 1 mg/mL stock solution.
