What is CD45 a marker of?
CD45 is a transmembrane protein tyrosine phosphatase located on most haematopoietic cells. It has several isoforms, and haematopoietic cells express one or more of the isoforms—CD45RO, CD45RA and CD45RB. CD45 immunoreactivity is recognised to be highly specific for non–Hodgkin’s lymphomas.
What is congenic marker?
Marker assisted breeding or marker assisted selection breeding, also known as “speed congenics” permits the production of congenic strains equivalent to 10 backcross generations in as few as five generations (Markel et al., 1997; Wakeland et al., 1997). …
What is CD45 1 a marker for?
1 and CD45. 2, have been established as a marker system to track haematopoietic cells following congenic mouse bone marrow transplants. Despite the frequent use of this model for studying the impact of genetic modifications on relative differentiation potential, it is now evident that a bias exists in CD45.
What are CD45 1 mice?
CD45 itself is a protein tyrosine phosphatase receptor expressed on all hematopoietic cells except mature erythrocytes and platelets and encoded by the Ptprc gene. Wild-type C57BL/6 mice normally carry the Ptprcb (CD45.
What is the difference between CD45 1 and CD45 2?
1 and CD45. 2 were more equipped to fight lethal doses of MCMV and influenza virus, respectively, while both mouse strains were equally resistant to C. rodentium infection. Detailed analysis of CD45.
Which cells are CD45+?
CD45 antigen (leukocyte common antigen), a unique and ubiquitous membrane glycoprotein with a molecular mass of about 200 kDa, is expressed on almost all hematopoietic cells except for mature erythrocytes.
What CD45 2?
CD45.2 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes.
What cells are CD45 +?
Are plasma cells CD45+?
The mean bone marrow plasma cell content by flow cytometry was 22% (median, 14%; range, 0.2–91%). In all, 30% of plasma cells expressed CD45 by flow cytometry (median, 14%; range, 0.1–100%). Among all patients, 44% (33/75) had more than 20% of plasma cells expressing CD45 and were categorized as the CD45+ group.
Are T cells CD45 positive?
In aggregate, CD45 is required for T cell function; however, differing experimental observations suggest that it can positively or negatively affect TCR signaling.
Is CD45 on T cells?
CD45 is a receptor-type protein tyrosine phosphatase (RT-PTP) that is among the most abundant proteins within the T cell plasma membrane, yet its role in regulating T cell function remains enigmatic (23). CD45 is required for TCR signaling because it activates Lck, which is required to phosphorylate the TCR complex.
How is the CD45 congenic marker system used?
The CD45 congenic marker system is a highly utilized technique to track hematopoietic cells following bone marrow transplantation (BMT), with CD45.1 and CD45.2 being efficiently distinguished by flow cytometry.
How are CD45.2 cells reconstituted in bone marrow?
We performed bone marrow transplants with wild-type CD45.1 and CD45.2 donor cells, and characterised haematopoietic cell reconstitution in dual-expressing CD45.1/2 host mice. We report an increase in CD45.2 reconstitution in the bone marrow that persists in the spleen, thymus and blood.
Where is the CD45 gene located in the cell?
CD45, also known as protein tyrosine phosphatase, receptor type C, is encoded by the Ptprc gene and is expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets 1.
What kind of leukocytes are positive for CD45.1?
CD45 + leukocytes from donor mice were found to be uniformly positive for either CD45.1 or CD45.2, whereas leukocytes from CD45.1/2 hosts were exclusively dual-expressing CD45.1 + /CD45.2 + (Supplementary Figure S1 ).
