What is the source of PstI?
Product Source A E. coli strain that carries the PstI gene from Providencia stuartii 164 (ATCC 49762).
How will you describe the action of PstI?
Description. Thermo Scientific PstI restriction enzyme recognizes CTGCA^G sites and cuts best at 37°C in O buffer (Isoschizomers: BspMAI). See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes.
How many fragments did the restriction enzyme PstI produce?
c) For those two restriction endonucleases, each DNA fragment in the single digest is cut by PstI into two DNA fragments in the double digest (i.e. the restriction endonuclease plus PstI).
What does the EcoRI PstI enzyme mixture do?
Restriction enzymes work by recognizing a particular sequence of bases on the DNA. The enzyme then cuts the backbones of both strands, allowing the DNA to separate into two pieces. For example, the enzyme EcoRI (see the figure, left, top) binds to the recognition sequence GAATTC and cuts between the G and the A.
Is pstl a restriction enzyme?
PstI is a useful enzyme for DNA cloning as it provides a selective system for generating hybrid DNA molecules. These hybrid DNA molecules can be then cleaved at the regenerated PstI sites. It is also an isoschizomer restriction enzyme SalPI from Streptomyces albus P.
How many fragments are produced by BamHI?
BamHI cleaves the same molecule into fragments of sizes 6.0, 10.1, and 12.9, and the label is found associated with the 6.0 and 10.1 fragments. When EcoRI and BamHI are used together, fragments of sizes 1.0, 2.0, 2.9, 3.5, 6.0, 6.2, and 7.4 kb are produced.
What is pst1 restriction enzyme?
PstI is a restiction enzyme used in molecular biology applications to cleave DNA at the recognition sequence 5′-CTGCA/G-3′, generating DNA fragments with 3′- cohesive termini.
Is pst1 sticky or blunt?
Function. PstI cleaves DNA at the recognition sequence 5′-CTGCA/G-3′ generating fragments with 3′-cohesive termini. This cleavage yields sticky ends 4 base pairs long. PstI is catalytically active as a dimer.
What is HaeIII restriction enzyme?
HaeIII is one of many restriction enzymes (endonucleases) a type of prokaryotic DNA that protects organisms from unknown, foreign DNA. It is a restriction enzyme used in molecular biology laboratories. It was the third endonuclease to be isolated from the Haemophilus aegyptius bacteria.
How many fragments are produced by HindIII?
four fragments
GGCC occurs, on average, every 44 bases, which is 0.256 kb. 3. You have a purified DNA molecule, and you wish to map restriction-enzyme sites along its length. After digestion with EcoRI, you obtain four fragments: 1, 2, 3, and 4.
What are restriction endonucleases?
Restriction endonucleases (REs) are bacterial enzymes that cleave double-stranded DNA. REs are present in bacteria presumably to destroy DNA from foreign sources (e.g., infecting bacteriophage) by cleaving the foreign DNA at specific recognition sites.
When to use Thermo Scientific PstI restriction enzyme?
Order requests are processed and shipped by your local distributor. Learn more Thermo Scientific PstI restriction enzyme recognizes CTGCA^G sites and cuts best at 37°C in O buffer (Isoschizomers: BspMAI).
Which is the restriction enzyme in Providencia stuartii 164?
PstI is a restriction enzyme purified from a recombinant E. coli strain that carries a plasmid encoding the PstI gene from Providencia stuartii 164. PstI cleaves the palindromic DNA sequence CTGCA ▼G. Concentration: 10U/µL.
What kind of restriction endonuclease is PstI?
PstI is a type II restriction endonuclease isolated from the Gram negative species, Providencia stuartii.
What is the storage buffer For PstI enzyme?
After 10-fold overdigestion with PstI, greater than 95% of the DNA fragments can be ligated and recut with this enzyme. Storage buffer: PstI is supplied in 50 mM KCl, 10 mM Tris HCl (pH 7.4), 0.1 mM EDTA, 1 mM dithiothreitol, 500 μg/ml BSA and 50% glycerol. Inactivation: Heat to 80 °C for 20 minutes.
